Search results for " Mannose-Binding Lectin"

showing 5 items of 5 documents

Increased susceptibility of complement factor B/C2 double knockout mice and mannan-binding lectin knockout mice to systemic infection with Candida al…

2008

Candida albicans is the major cause of systemic fungal infections in immunocompromised patients. We investigated the susceptibility of mice deficient in complement factor B and C2 (Bf/C2-/-), C1q (C1qa-/-), and mannan-binding lectin (MBL)-A (MBL-A) and MBL-C (MBL-A/C-/-) to systemic infection with C. albicans. Animals were infected i.p. with 10(8)C. albicans blastoconidia and monitored for mortality. Bf/C2-/- mice showed high mortality (over 90%) within the study period of 3 weeks. In contrast, mortality in C1qa-/- mice was below 15% whereas that of MBL-A/C-/- mice was 40% (P0.001). Intravenous infection of mice with 8x10(5) blastoconidia resulted in the same trend with Bf/C2-/- mice being …

Immunologychemical and pharmacologic phenomenaOpportunistic InfectionsMannose-Binding LectinBlastoconidiumComplement factor BMicrobiologyMicePhagocytosisSpecies SpecificityCandida albicansAnimalsGenetic Predisposition to DiseaseCandida albicansDouble knockoutComplement ActivationMolecular BiologyMannan-binding lectinMice KnockoutbiologyCandidiasisLectinComplement Pathway Mannose-Binding LectinComplement C2bacterial infections and mycosesbiology.organism_classificationCorpus albicansKnockout mousebiology.proteinComplement Factor BMolecular Immunology
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Functional analysis of the classical, alternative, and MBL pathways of the complement system: standardization and validation of a simple ELISA.

2004

Primary defence against invading microorganisms depends on a functional innate immune system and the complement system plays a major role in such immunity. Deficiencies in one of the components of the complement system can cause severe and recurrent infections, systemic diseases, such as systemic lupus erythematosus (SLE) and renal disease. Screening for complement deficiencies in the classical or alternative complement pathways has mainly been performed by haemolytic assays. Here, we describe a simple ELISA-based format for the evaluation of three pathways of complement activation. The assays are based on specific coatings for each pathway in combination with specific buffer systems. We ha…

Innate immune systemSystemic lupus erythematosusImmunologyComplement Pathway AlternativeComplement Pathway Mannose-Binding LectinEnzyme-Linked Immunosorbent AssayComplement System ProteinsBiologyComplement fixation testmedicine.diseaseMannose-Binding LectinComplement systemComplement (complexity)Immune systemImmunologymedicineImmunology and AllergyHumansLupus Erythematosus SystemicComplement Pathway ClassicalReagent Kits DiagnosticFicolinComplement ActivationMannan-binding lectinJournal of immunological methods
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New functional ligands for ficolin-3 among lipopolysaccharides of Hafnia alvei.

2011

Ficolin-1 (M), ficolin-2 (L), ficolin-3 (H) and mannan-binding lectin (MBL) activate the complement system and have opsonic activity. The specificity of ficolin-3 is poorly characterized and currently limited to a few ligands only. We present new specific targets for human ficolin-3, identified among lipopolysaccharides (LPSs, endotoxin) of Hafnia alvei. The interaction was restricted to LPSs of four strains: 23, Polish Collection of Microorganisms (PCM) 1200, PCM 1203 and PCM 1205 and limited to their O-specific polysaccharides (O-specific PSs) composed of different numbers of oligosaccharide (OS) repeating units (RUs). Moreover, these LPS/ficolin-3 complexes activated the lectin pathway o…

LipopolysaccharidesDisaccharideLigandsBiochemistrychemistry.chemical_compoundLectinsAnimalsHumansBovine serum albuminOpsoninchemistry.chemical_classificationbiologyLectinO AntigensComplement Pathway Mannose-Binding LectinHafnia alveiSerum Albumin BovineOligosaccharideComplement systemEndotoxinschemistryBiochemistryLectin pathwaybiology.proteinCattleFicolinGlycobiology
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Involvement of complement pathways in patients with bacterial septicemia.

2007

The complement system is a major humoral portion of the innate immune system, playing a significant role in host defence against microorganisms. The biological importance of this system is underlined by the fact that at least three different pathways for its activation exist, the classical, the MBL and the alternative pathway. To elucidate the involvement of the classical and/or the MBL pathway during bacterial septicemia, 32 patients with gram-positive and 30 patients with gram-negative bacterial infections were investigated. In patients with gram-positive bacteria, a significant consumption of C1q (p=0.005) but not of mannose-binding lectin (MBL) (p=0.2) was found during the acute phase o…

MESH: Complement Pathway Mannose-Binding LectinLipopolysaccharidesSalmonellaMESH: Complement C1qLipopolysaccharideImmunologychemical and pharmacologic phenomenaBacteremiamedicine.disease_causeGram-Positive BacteriaMannose-Binding LectinMicrobiologyMESH: Gram-Positive Bacteria03 medical and health scienceschemistry.chemical_compoundClassical complement pathway0302 clinical medicinemedicineHumans[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyComplement Pathway ClassicalMESH: BacteremiaMolecular Biology030304 developmental biology0303 health sciencesInnate immune systemMESH: HumansbiologyComplement C1qLectinSalmonella entericaComplement Pathway Mannose-Binding LectinMESH: Complement Pathway Classicalbiology.organism_classificationbacterial infections and mycoses3. Good healthComplement systemMESH: Mannose-Binding LectinchemistryMESH: Salmonella entericaImmunologyAlternative complement pathwaybiology.proteinMESH: LipopolysaccharidesBacteria030215 immunologyMolecular immunology
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'Immunogenetics of Aging': report on the activities of the 15th International HLA and Immunogenetics Working Group and 15th International HLA and Imm…

2011

'Immunogenetics of Aging' is a component that was first included in the 14th International HLA and Immunogenetics Workshop (IHIWS) and developed further within the 15th Workshop. The aim of this component was to assess the impact of human leukocyte antigen (HLA) genes, cytokine genes, and some innate immunity genes such as killer-cell immunoglobulin-like receptors (KIRs) and mannose-binding lectin 2 (MBL2) in successful aging and their contribution to the better understanding of immune dysfunction in old age. Within the 15th IHIWS new populations were included in the analysis. Additional cytokine gene polymorphisms were assessed and innate immunity genes were analyzed for possible relevance…

aging; cytokine gene polymorphism; killer-cell immunoglobulin-like receptor genes; longevity; mannose-binding lectin 2 geneGeneticsInnate immune systemSuccessful agingkiller-cell immunoglobulin-like receptor genemedia_common.quotation_subjectImmunologyHaplotypeagingLongevityKILLER-CELL IMMUNOGLOBULIN-LIKE RECEPTOR GENESGeneral MedicineImmunogeneticsHuman leukocyte antigenBiologyCytokine gene polymorphismmannose-binding lectin 2 geneBiochemistryImmune systemImmunologyGeneticsImmunology and AllergyLONGEVITYGenemedia_common
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